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Genome-enabled Studies of Anaerobic, Nitrate-Dependent Iron Oxidation in the Chemolithoautotrophic Bacterium Thiobacillus denitrificans

Source:  Dan Hawkes

FIGURE 5. Expression profiles and annotations for a group of 16 of the most highly expressed genes under under nitrate-dependent UO2-, FeCO3-, and Fe2+-oxidizing conditions (Groups VI, V, and II, respectively; see text). The two genes highlighted in blue were targeted for mutation studies. The intensity color scale is the same as for Figure 2.
Thiobacillus denitrificans is a chemolithoautotrophic bacterium capable of anaerobic, nitrate-dependent U(IV) and Fe(II) oxidation, both of which can strongly influence the long-term efficacy of in situ reductive immobilization of uranium in contaminated aquifers. In a study published recently in Frontiers in Microbiology, ESD’s Harry Beller and Peng Zhu (and others) report on efforts to identify genes associated with nitrate-dependent Fe(II) oxidation. Overall, their results indicate that nitrate-dependent Fe(II) oxidation in T. denitrificans is not catalyzed by the same c-type cytochromes involved in U(IV) oxidation, nor have other c-type cytochromes yet been implicated in the process.

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Citation: Beller, H.R., P. Zhou, T.C. Legler, A. Chakicherla, S. Kane, T.E. Letain, and P.A. O’Day (2013), Genome-enabled studies of anaerobic, nitrate-dependent iron oxidation in the chemolithoautotrophic bacterium Thiobacillus denitrificans. Frontiers in Microbiology, DOI: 10:3389/fmicb.2013.00249.

Funding: BER SBR SFA